Search results for "Stromal vascular fraction"

showing 5 items of 5 documents

Adipose Stromal Vascular Fraction Improves Cardiac Function in Chronic Myocardial Infarction Through Differentiation and Paracrine Activity

2012

Fresh adipose-derived cells have been shown to be effective in the treatment of acute myocardial infarction (MI), but their role in the chronic setting is unknown. We sought to determine the long-term effect of the adipose derived-stromal vascular fraction (SVF) cell transplantation in a rat model of chronic MI. MI was induced in 82 rats by permanent coronary artery ligation and 5 weeks later rats were allocated to receive an intramyocardial injection of 107 GFP-expressing fresh SVF cells or culture media as control. Heart function and tissue metabolism were determined by echocardiography and 18F-FDG-microPET, respectively, and histological studies were performed for up to 3 months after t…

Cardiac function curvemedicine.medical_specialtymedicine.medical_treatmentHeart VentriclesBiomedical EngineeringMyocardial Infarctionlcsh:MedicineAdipose tissue030204 cardiovascular system & hematologyRevascularizationRats Sprague-Dawley03 medical and health sciences0302 clinical medicineFibrosisInternal medicineParacrine CommunicationmedicineAdipocytesMyocardial RevascularizationAnimalsMyocardial infarctionAngiogenic ProteinsVentricular remodeling030304 developmental biology0303 health sciencesTransplantationTissue Inhibitor of Metalloproteinase-1Ventricular Remodelingbusiness.industrylcsh:RCell DifferentiationTissue Inhibitor of MetalloproteinasesCell BiologyStromal vascular fractionmedicine.diseaseRatsTransplantationDisease Models AnimalPhenotypeEchocardiographyPositron-Emission TomographyChronic DiseaseCardiologyCytokinesFemaleStromal CellsbusinessCell Transplantation
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Evaluation of the stromal vascular fraction of adipose tissue as the basis for a stem cell-based tissue-engineered vascular graft

2017

Abstract Objective One of the rate-limiting barriers within the field of vascular tissue engineering is the lengthy fabrication time associated with expanding appropriate cell types in culture. One particularly attractive cell type for this purpose is the adipose-derived mesenchymal stem cell (AD-MSC), which is abundant and easily harvested from liposuction procedures. Even this cell type has its drawbacks, however, including the required culture period for expansion, which could pose risks of cellular transformation or contamination. Eliminating culture entirely would be ideal to avoid these concerns. In this study, we used the raw population of cells obtained after digestion of human lipo…

Adult0301 basic medicinePathologymedicine.medical_specialtyTime FactorsCellular differentiationMyocytes Smooth MusclePopulationAdipose tissueCell Separation030204 cardiovascular system & hematologyMesenchymal Stem Cell TransplantationMuscle Smooth VascularArticleBlood Vessel Prosthesis Implantation03 medical and health sciences0302 clinical medicineLipectomyCell MovementBlood vessel prosthesisAnimalsHumansMedicineAorta AbdominaleducationCells CulturedBioprosthesiseducation.field_of_studyTissue EngineeringTissue Scaffoldsbusiness.industryAngiotensin IIMesenchymal stem cellCell DifferentiationMesenchymal Stem CellsAnatomyStromal vascular fractionAngiotensin IIBlood Vessel ProsthesisPhenotype030104 developmental biologyAdipose TissueRats Inbred LewFemaleSurgeryStromal CellsStem cellbusinessCardiology and Cardiovascular Medicine
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Regenerative Capacity of Adipose Derived Stem Cells (ADSCs), Comparison with Mesenchymal Stem Cells (MSCs).

2019

Adipose tissue is now on the top one of stem cell sources regarding its accessibility, abundance, and less painful collection procedure when compared to other sources. The adipose derived stem cells (ADSCs) that it contains can be maintained and expanded in culture for long periods of time without losing their differentiation capacity, leading to large cell quantities being increasingly used in cell therapy purposes. Many reports showed that ADSCs-based cell therapy products demonstrated optimal efficacy and efficiency in some clinical indications for both autologous and allogeneic purposes, hence becoming considered as potential tools for replacing, repairing, and regenerating dead or dama…

bone marrowmedicine.medical_treatmentAdipose tissueregenerative medicineBone Marrow CellsReviewMesenchymal Stem Cell TransplantationRegenerative medicinestem cell therapyCatalysisUmbilical CordInorganic ChemistryCell therapylcsh:ChemistryADSCsMedicineAnimalsHumansPhysical and Theoretical ChemistryMolecular Biologylcsh:QH301-705.5Spectroscopymesenchymal stem cellsbusiness.industryOrganic ChemistryMesenchymal stem cellCell DifferentiationGeneral MedicineStem-cell therapyStromal vascular fractionComputer Science Applicationsadipose tissueadipose derived stem cellsmedicine.anatomical_structurelcsh:Biology (General)lcsh:QD1-999Cancer researchBone marrowStem cellbusinessInternational journal of molecular sciences
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Combined platelet-rich plasma and lipofilling treatment provides great improvement in facial skin-induced lesion regeneration for scleroderma patient…

2017

Background The use of stem cells, including mesenchymal stem cells (MSCs), for regenerative medicine is gaining interest for the clinical benefits so far obtained in patients. This study investigates the use of adipose autologous tissue in combination with platelet-rich plasma (PRP) to improve the clinical outcome of patients affected by systemic sclerosis (SSc). Methods Adipose-derived mesenchymal stem cells (AD-MSCs) and PRPs were purified from healthy donors and SSc patients. The multilineage differentiation potential of AD-MSCs and their genotypic–phenotypic features were investigated. A cytokine production profile was evaluated on AD-MSCs and PRPs from both healthy subjects and SSc pat…

0301 basic medicineMalePathologyCell- and Tissue-Based TherapyAdipose tissueMedicine (miscellaneous)Gene ExpressionRegenerative MedicineCell therapyCell therapySystemic sclerosiAdipose-derived mesenchymal stem cells; Cell therapy; Lipofilling; Mesenchymal stem cells; Platelet-rich plasma; Regenerative medicine; Systemic sclerosis; Medicine (miscellaneous); Molecular Medicine; Biochemistry Genetics and Molecular Biology (miscellaneous); Cell Biologylcsh:QD415-436skin and connective tissue diseasesMesenchymal stem cellSkinAged 80 and overlcsh:R5-920integumentary systemCell DifferentiationStromal vascular fractionMiddle Agedmedicine.anatomical_structureAdipose TissueMolecular MedicineCytokinesSystemic sclerosisFemaleStem celllcsh:Medicine (General)Adultmedicine.medical_specialtyPrimary Cell CultureConnective tissueNeovascularization PhysiologicMesenchymal Stem Cell TransplantationBiochemistry Genetics and Molecular Biology (miscellaneous)lcsh:Biochemistry03 medical and health sciencesPlatelet-rich plasmaAntigens CDAdipose-derived mesenchymal stem cellsmedicineHumansCell ProliferationAdipose-derived mesenchymal stem cellLipofillingScleroderma Systemicbusiness.industryRegeneration (biology)ResearchMesenchymal stem cellMesenchymal Stem CellsCell Biology030104 developmental biologyPlatelet-rich plasmaImmunologybusinessStem cell researchtherapy
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Magnetic resonance and ultrasound in achilles tendinopathy: Predictive role and response assessment to platelet-rich plasma and adipose-derived strom…

2017

To assess the correlation between magnetic resonance and ultrasound findings and clinical outcome after intratendinous injection of leucocyte-rich platelet-rich plasma or adipose-derived stromal vascular fraction in patients with non-insertional Achilles tendinopathy.Forty-three patients (age: 47.8±5.1, range 29-55) with unilateral or bilateral non-insertional Achilles tendinopathy (58 tendons overall) were randomly assigned to platelet-rich plasma (22 patients, 28 tendons) or adipose-derived stromal vascular fraction (21 patients, 30 tendons) injection group. All patients underwent magnetic resonance (tendon cross-sectional area, signal intensity, maximum anteroposterior thickness were mea…

AdultMalemedicine.medical_specialtyMagnetic Resonance SpectroscopyIntraclass correlationVisual analogue scaleAdipose-tissue stromal vascular fractionAchilles TendonInjections030218 nuclear medicine & medical imaging03 medical and health sciencesMagnetic resonance imaging0302 clinical medicineVisual analogue scaleNuclear Medicine and ImagingUltrasoundHumansMedicineRadiology Nuclear Medicine and imagingObesityPain MeasurementUltrasonographyAnalysis of Variance030222 orthopedicsAchilles tendonmedicine.diagnostic_testPlatelet-Rich Plasmabusiness.industryUltrasoundMagnetic resonance imagingGeneral MedicineMiddle AgedStromal vascular fractionmedicine.diseaseTendonTreatment Outcomemedicine.anatomical_structureAdipose TissueTendinopathyFemaleRadiologyStromal CellsTendinopathyRadiologybusinessAdipose-tissue stromal vascular fraction; Magnetic resonance imaging; Platelet-rich plasma; Ultrasound; Visual analogue scale; Radiology Nuclear Medicine and ImagingEuropean Journal of Radiology
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